发明名称 |
VERFAHREN ZUM KLASSIFIZIEREN VON LEUKOZYTEN UND REAGENZIEN |
摘要 |
A reagent for the classification and counting of leucocytes, contains a surfactant of formula (I) and/or a surfactant of formula (II): (where R1 is 10-22C alkyl, alkenyl or alkynyl; R2 is O, -COO- or a group of formula (III); n is 8-30; X is -SO3Na, -COONa, -OSO2ONa or -ONa) and pref. also contains an osmotic pressure raising agent and a solubilising agent. By use of different solubilising agents the leucocytes can be classified into five groups of lymphocytes, monocytes and granulocytes. The solubilising agents for the five groups are: (1) urea, thiourea, N,N-dimethylurea, N,N'-dimethylurea, ethyleneurea, methylurethane, or urethane; (2) octyl beta-D-glucoside, CHAPS, CHAPSO, MEGA-8, MEGA-9, MEGA-10, sucrose monocaproate, or N-formylmethyl leucylalanine; (3) guanidine thiocyanate, hydrochloride, nitrate, rhodanide, carbonate, sulphate or phosphate or guanidylguanidine or 1,1,3,3-tetraguainidie; (4) sodium deoxycholate, cholic acid or taurocholic acid; (5) sodium mono-, di- or trichloroacetate or mono-, di- or tribromoacetate. |
申请公布号 |
DE3855153(D1) |
申请公布日期 |
1996.05.02 |
申请号 |
DE19883855153 |
申请日期 |
1988.05.27 |
申请人 |
TOA MEDICAL ELECTRONICS CO. LTD., KOBE, HYOGO, JP |
发明人 |
HAMAGUCHI, YUKIO, HYOGO-KU KOBE-SHI HYOGO 652, JP;ITO, KENJI TOA MEDICAL ELECTRONICS CO., LTD., HYOGO-KU KOBE-SHI HYOGO 652, JP;TSUJINO, YUKIO TOA MEDICAL ELECTRONICS CO., LTD., HYOGO-KU KOBE-SHI HYOGO 652, JP;MORIYAMA, KAZUHIRO TOA MED. ELECTR. CO. LTD, HYOGO-KU KOBE-SHI HYOGO 652, JP;TAKENAKA, IKUYA TOA MED. ELECTR. CO. LTD., HYOGO-KU KOBE-SHI HYOGO 652, JP;MORIKAWA, TAKASHI TOA MED. ELECTR. CO. LTD., HYOGO-KU KOBE-SHI HYOGO 652, JP;OHMI, HITOMI TOA MED. ELECTR. CO. LTD., HYOGO-KU KOBE-SHI HYOGO 652, JP |
分类号 |
H01H11/00;G01N33/48;G01N33/49;G01N33/50;H01H15/04 |
主分类号 |
H01H11/00 |
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