| 摘要 |
Self-priming, a simple and rapid method for amplification of non-characterised DNA, comprises: (a) carrying out second-strand synthesis at about 20-37 deg C to favour formation of hairpin structures (4) in the newly formed strand, (b) continuing synthesis of the second strand up to the initial sequence, corresponding to the primer region (5) and (c) amplifying the target sequence with only one adjacent, specific primer.
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| 申请人 |
PACHMANN, KATHARINA, DR., 95448 BAYREUTH, DE;GOEHLY, URSULA, 83700 ROTTACH-EGERN, DE |
发明人 |
PACHMANN, KATHARINA, DR., 95448 BAYREUTH, DE;GOEHLY, URSULA, 83700 ROTTACH-EGERN, DE |
