| 摘要 |
Diagnostic, radiotherapy, and chemotherapy methods which may be used in conjunction with gene therapy techniques, and the use of certain compounds in performing these methods. The methods are applicable to populations of cells into which a foreign gene has been transferred, which foreign gene expresses a protein which preferably is not naturally occuring within the cells. A compound is selected which will interact selectively with the protein expressed by the foreign gene to produce a product which is trapped within the cells, is cytotoxix or cytostatic to the cells, or both, depending upon whether the compound is being used for diagnostic purposes or for radiotherapy or chemotherapy purposes. In the case of diagnostic applications, trapping of the product, which is labelled, permits the product to accumulate in those of the cells in which the protein has been expressed by the foreign gene, thus facilitating detection of the labelled product in those cells. In the case of radiotherapy applications, trapping of the product, which is radioactive as a result of the compound being radiolabelled, permits the product to accumulate in those of the cells in which the protein has been expressed by the foreign gene, thus facilitating radiotherapeutic effects directed specifically at those cells. In the case of chemotherapy applications, interaction of the protein with the compound has either a cytotoxic or a cytostatic effect on the cells, which is enhanced if the product is trapped within those of the cells in which the protein has been expressed.
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