发明名称 |
A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification, a diagnostic assay employing said process, a kit and a container suitable for carrying out said process or assay |
摘要 |
A process for making the product of a nucleic acid amplification reaction, which amplification reaction employs one or more primer pairs, incapable of being a target for further amplification comprising: contacting the amplified product with an oligonucleotide capable of hybridizing to a stretch a nucleotides of the amplified product, said stretch being situated between the hybridisation sites of a pair of primers, under conditions which allow formation of a hybridisation complex between the oligonucleotide and the amplified product to occur, said oligonucleotide being modified in such a way that it protects the double stranded part of the hybridisation complex hybridized to the oligonucleotide from degradation, and subjecting the hybridisation complex to a degradative treatment under circumstances such that at least the part of the hybridisation complex capable of hybridizing to the primers is degraded. An assay for diagnosing the presence of a nucleic acid sequence in a sample comprising carrying out the afore-mentioned method and a kit for carrying out the afore-mentioned method. |
申请公布号 |
EP0709468(A1) |
申请公布日期 |
1996.05.01 |
申请号 |
EP19950202261 |
申请日期 |
1995.08.22 |
申请人 |
AKZO NOBEL N.V. |
发明人 |
VAN GEMEN, BOB;SCHUKKINK, ADRIANA FREDERIEKE |
分类号 |
G01N33/50;C07H21/00;C07H21/04;C12M1/00;C12N15/09;C12P19/34;C12Q1/68 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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