Method for coupling antibody to latex substrate and its use in immunoassay

摘要

Antibody-latex reagents are prepared that utilize a novel site-specific covalent linkage of antibodies, via their interfering Fc moieties, onto novel polymeric latex sphere substrates, thereby preserving the antigen binding sites of the antibodies. This immobilization of antibodies is essential for high specific activity and sensitivity assays, and is also economical and much simpler than other covalent immobilizations.