Oligonucleotide-linked magnetic particles and uses thereof

摘要

Monodisperse, superparamagnetic particles carrying a plurality of molecules of an oligonucleotide are disclosed and may be used inter alia for sequencing single-stranded nucleic acids. The oligonucleotide may be covalently attached or affinity bonded to the particles either by their 3' or 5' termini. The particles have a specific gravity in the range 1.1-1.8 and are 1-10 microns in diameter. A kit for the isolation or processing of target nucleic acid is also disclosed.