A device for collecting uncontaminated urine specimens from adult females, adult uncircumcised males and pediatric males. The device includes an elongated flexible tubular conduit member having a funnel-shaped opening at one end to be in sealing relationship with a urethra or glans penis and a discharge end to be received within a sample container. The sample container includes an opening with sealing means therein to receive the discharge end of the elongated flexible tubular conduit member in an open position to receive a urine specimen and in a closed position when the discharge end of the elongated flexible tubular conduit member is withdrawn.