Glycolipid ceramide deacylase

摘要

The present invention provides a glycolipid ceramide deacylase enzyme having a wide substrate specificity, in particular, acting well on neutral glycolipids. The invention also provides a method of producing a lysosphingolipid using the above enzyme. The glycolipid ceramide deacylase is characterized by: acting on a ceramide in the molecule of a sphingoglycolipid and hydrolyzing it into a sphingosine base and a fatty acid, thus forming a lysoglycolipid and a fatty acid; having a substrate specificity of acting on neutral glycolipids and acidic glycolipids; having an optimum pH value range of from 5 to 7; having an optimum temperature of about 40 DEG C; having a pH stability of being stable within a pH value range of from 4 to 9, when treated at 5 DEG C for 16 hours; and having a heat stability of being stable, when treated at 60 DEG C for 30 minutes. A lysosphingolipid can be produced by treating a sphingolipid with the above enzyme.