发明名称 |
BIOSENSOR WITH BIOMATERIAL COVALENTLY FIXED ON A SIGNAL-ACTIVE SURFACE |
摘要 |
The successful bonding of bioactive material, especially enzymes, to the signal-active surface of a biosensor can be achieved by means of a heterobifunctional crosslinking agent having a group A reactive for the signal-active surface, e.g. a group reacting with -OH, -SH or -NHx, and a photoactivatable, biomaterial-reactive group B. Known groups B are, in particular, azidophenyl and azidosalicylic groups. Crosslinking agents containing such groups B on the one hand and succinimide ester groups on the other are commercially available and hence particularly attractive. The signal-active surface can be especially a Si3N4 surface with reactive NHx groups, as can be obtained from the gas phase by SiH4/NH3 reaction, especially on a fresh SiO2 surface, and has been freed from oxides especially by treatment with dilute hydrofluoric acid.
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申请公布号 |
CA2202043(A1) |
申请公布日期 |
1996.04.18 |
申请号 |
CA19952202043 |
申请日期 |
1995.09.30 |
申请人 |
FORSCHUNGSZENTRUM JUELICH GMBH |
发明人 |
THUST, MARION;KORDOS, PETER;KAUPP, ULRICH BENJAMIN;VETTER, JOACHIM;SCH NING, MICHAEL JOSEF;LUETH, HANS |
分类号 |
C12M1/40;C12Q1/00;C12Q1/25;G01N27/327;G01N33/543;(IPC1-7):C12M1/40;G01N27/26;C12M1/34;G01N33/552 |
主分类号 |
C12M1/40 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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