| 摘要 |
<p>PROBLEM TO BE SOLVED: To obtain a new immunoconjugate capable of inducing cytotoxicity and chemotactic activity and suitable for a targeted tumor therapy. SOLUTION: This immunoconjugate is composed of a monoclonal antibody specific for a tumor cell having an antigen epitope of epidermal growth factor receptor(EGFR) or a fragment thereof, and a chemokine protein fusible to the antibody or the antibody fragment, preferably one selected from the C-X-C family, especially IL-δ. The immunoconjugate links to an EGFR-positive cell to induce chemotactic activity, the release of myeloperoxidase and superoxide, and in-situ tumor lysis. Thus, the immunoconjugate is suitable for tumor treatment. The immunoconjugate is obtained as follows: DNA sequences, which encode the antibody or the antibody fragment and a ligand having a biological activity, respectively, are fused on a single strand DNA by using oligonucleotide which is supplementary to the desired fused DNA sequence; the obtained fused substance is transduced into an expression vector to transform the host cell; and the transformed host cell is cultured in a nutrient solution to express the fused protein.</p> |
