发明名称 |
In vitro phage lambda DNA packaging system |
摘要 |
A bacterial preparation capable of packaging phage lambda DNA is disclosed. This preparation is in lyophilized form and is stable at ambient temperature. In a preferred form, the preparation contains an over-expressed terminase protein, is prepared from the bacterial strain E. coli Cla [ lambda c/857 Sam7 DELTA (cos-Nu1-A)::Knr]/ lambda pTER and is capable of a packaging efficiency of at least 1x108 pfu/ mu g wild type lambda DNA. The present invention is also a method of creating a phage lambda DNA packaging extract comprising the steps of preparing a bacterial extract capable of packaging phage lambda and lyophilizing said extract.
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申请公布号 |
US5508187(A) |
申请公布日期 |
1996.04.16 |
申请号 |
US19930173743 |
申请日期 |
1993.12.23 |
申请人 |
PHARMACIA P-L BIOCHEMICALS, INC. |
发明人 |
FRACKMAN, SUSAN G.;FRANCISKOVICH, PHILLIP P.;JOLLY, JAMES F.;LUHM, ROBERT A.;RIEDL, WILLIAM A. |
分类号 |
C12N7/01;C12N9/22;C12N15/10;(IPC1-7):C12N1/20;C12N7/00;C12N15/09 |
主分类号 |
C12N7/01 |
代理机构 |
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主权项 |
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地址 |
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