摘要 |
The successful bonding of bioactive material, especially enzymes, to the signal-active surface of a biosensor can be achieved by means of a heterobifunctional crosslinking agent having a group A reactive for the signal-active surface, e.g. a group reacting with -OH, -SH or -NHx, and a photoactivatable, biomaterial-reactive group B. Known groups B are, in particular, azidophenyl and azidosalicylic groups. Crosslinking agents containing such groups B on the one hand and succinimide ester groups on the other are commercially available and hence particularly attractive. The signal-active surface can be especially a Si3N4 surface with reactive NHx groups, as can be obtained from the gas phase by SiH4/NH3 reaction, especially on a fresh SiO2 surface, and has been freed from oxides especially by treatment with dilute hydrofluoric acid.
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申请人 |
FORSCHUNGSZENTRUM JUELICH GMBH, 52428 JUELICH, DE |
发明人 |
THUST, MARION, 50939 KOELN, DE;SCHOENING, MICHAEL JOSEF, DR., 52428 JUELICH, DE;VETTER, JOACHIM, DR., 50678 KOELN, DE;KAUPP, ULRICH BENJAMIN, PROF. DR., 52062 AACHEN, DE;KORDOS, PETER, PROF. DR., 52428 JUELICH, DE;LUETH, HANS, PROF. DR., 52076 AACHEN, DE |