| 摘要 |
Procedure for releasing poly-3-hydroxy carboxylic acid granules from cells of Gram-negative bacteria containing these by expression of a cloned natural or chimeric lysis gene, in which before induction of lysis gene expression the ionic strength of doubly charged cations is increased to 0.05 to 0.5 mol, expression of the lysis gene is induced by increasing the temperature, the cells are harvested and the still moist cells are resuspended in water or buffer solutions, whereupon the polymer granules are released by spontaneous lysis. |
