TOPOGRAPHIC GENOTYPING

摘要

Method of topographic genotyping which comprises placing a biological specimen having DNA of a patient under a microscope, inspecting said specimen and choosing a microscopic-sized target based on its histopathologic characteristics. The target is then separated from the specimen and its DNA is isolated, amplified, and assayed for the presence of mutations. The present invention also pertains to a method of topographic genotyping which comprises the step of separating a section from a specimen of fixative-treated tissue, obtaining DNA from said section and amplifying the DNA by PCR using cycles which comprise heating to a temperature no greater than 99 DEG C and then cooling to a temperature of 55 DEG C in 5 mininutes for each cycle. Sequences with said DNA are then assayed for the presence of mutations. Preferably, the separating step includes the step of cutting one to three 2-6 micron thick histologic sections from the specimen.