Biosensor for and method of electrogenerated chemiluminescent detection of nucleic acid adsorbed to a solid surface

摘要

Single-strand DNA was immobilized on an electrode covered with an aluminum alkanebisphosphonate film by immersing it in an ss-DNA solution. The immobilized ss-DNA labeled with Ru(bpy)3<2+> was detected by monitoring the electrogenerated chemiluminescence (ECL) produced upon oxidation in a solution containing tri-n-propylamine. After immobilization of unlabeled ss-DNA, a complementary labeled strand DNA was hybridized to produce ds-DNA on the surface. The extent of DNA hybridization was determined by ECL of the labeled Ru(bpy)3<2+>. Surface immobilized ds-DNA could also be detected by observing the ECL of intercalated Ru(phen)3<2+>. Transmission electron microscopy (TEM) was employed to image the film and the immobilized DNA.