发明名称 |
PROCESS FOR MANUFACTURE OF A MODIFIED COLLAGEN-INDUCED PLATELET AGGREGATION INHIBITOR PALLIDIPIN |
摘要 |
The invention provides a process for manufacture of a recombinant protein called Asp-Pallidipin. Asp-Pallidipin inhibits the collagen-induced platelet aggregation of mammalian platelets. The Asp-Pallidipin comprises: (i) a protein (Pallidipin) selected from the group of Pallipin proteins, and (ii) the amino acid aspartic acid, wherein the aspartic acid is connected by a peptide bond with the N-terminal end of Pallidipin. The process comprises the steps aa) transfecting at least one bacterium with an appropriate vector, wherein the vector comprises: (i) a DNA or cDNA coding for the recombinant AspPallidipin, (ii) a suitable signal peptide sequence which signal sequence is cleaved so that the amino acid aspartic acid is in the position +1 of the amino acid sequence seen from the position of the Pallidipin and (iii) a suitable promoter; bb) expressing the preprotein comprising Asp-Pallidipin and the signal sequence; cc) transporting the Asp-Pallidipin from the cytoplasm of the bacterium to the periplasm, cleavage of the preprotein by at least one protease during the transport, producing the Asp-Pallidipin, dd) isolating the Asp-Pallidipin by extracting the periplasm, and ee) purifying the AspPallidipin. The protein is used as a medicament for inhibiting of collageninduced human platelet aggregation or of cancer with metastatic tumor cells.
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申请公布号 |
CA2199719(A1) |
申请公布日期 |
1996.03.21 |
申请号 |
CA19952199719 |
申请日期 |
1995.09.11 |
申请人 |
SCHERING AKTIENGESELLSCHAFT |
发明人 |
NOESKE-JUNGBLUT, CHRISTIANE;BECKER, ANDREAS;HAENDLER, BERNARD |
分类号 |
C12N15/09;A61K38/00;A61K38/17;A61P7/02;A61P9/10;A61P35/00;C07K14/435;C12N1/21;C12N15/12;C12N15/30;C12N15/62;C12P21/00;C12P21/02;C12R1/19;(IPC1-7):C12N15/12;C07K1/16 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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