摘要 |
PURPOSE: To efficiently obtain the L-tropolonyl alanine useful as an antimicrobial agent, an insecticide, a metabolism-antagonizing agent, etc., in a good yield by allowing a tyrosinephenol lyase to act on a substrate comprising tropolone, etc., in an aqueous medium. CONSTITUTION: This L-tropolonyl alanine is obtained by allowing a tyrosine phenol lyase such as Erwinia herbicola ATCC 21433 originated from Erwinia herbicola on a substrate comprising (A) tropolone and (B) (i) a fi-chloro-L-alanine such as β-chloro-L-alanine, (ii) pyruvic acid and ammonia, or (iii) a serine such as L-serine in an aqueous medium, and subsequently collecting the L-toropolonyl alanine produced in the aqueous medium. |