| 摘要 |
PURPOSE: To obtain a new endonuclease 1 which is a rare cutter, capable of cleaving specific sites of a specified double-stranded DNA sequence, having high thermostability and capable of greatly cleaving the DNA and facilitating the genetic manipulation. CONSTITUTION: This new endonuclease 1 is capable of cleaving sites indicated by arrows in a DNA sequence of formula I and producing a 3'-protruding cleaved site of 4 bases of AGAT or ATCT in its complementary chain and prepared from a superthermophilic primordial microorganism such as Pyrococcus furiosus KOD1 strain, has about 41kDa molecular weight and contains an amino acid sequence of formula II. Furthermore, the endonuclease 1 is obtained by culturing a recombinant host cell (e.g. Escherichia coli) transformed with a DNA recombinant vector having a DNA, capable of coding the endonuclease 1 and inserted into a vector. |
