发明名称 |
EXPRESSION ENHANCER AND USE THEREOF TO INCREASE THE YIELD INTHE EXPRESSION OF RECOMBINANT GENES |
摘要 |
An expression enhancer has a DNA sequence which is capable of forming a t-RNA clover-leaf structure after transcription and hybridizes in that region of the DNA which, after transcription, forms the anticodon loop in the clover-leaf structure with an oligonucleotide with the sequence 5'-GACTTAGAAGGTCGTT-3' or its complementary sequence (5'-AACGACCTTCTAAGTC-3'). It can be used to increase the yield in the expression of a recombinant gene by transformation of suitable host cells with an expression vector containing the recombinant gene, whereby it is likewise introduced into the host cells in a form capable of expression and expressed.
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申请公布号 |
CA2002515(C) |
申请公布日期 |
1996.03.19 |
申请号 |
CA19892002515 |
申请日期 |
1989.11.08 |
申请人 |
BOEHRINGER MANNHEIM GMBH |
发明人 |
BRINKMANN, ULRICH;MATTES, RALF;BUCKEL, PETER |
分类号 |
C07H21/04;C12N9/64;C12N9/72;C12N15/09;C12N15/49;C12N15/58;C12N15/67;C12N15/70;C12P21/00;C12R1/19;(IPC1-7):C12N15/67 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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