| 摘要 |
<p>The present invention relates to a novel expression system for recombinant fibrinogen, fibrinogen variants and subunits thereof, to yeasts transformed with the expression system, to the use of the expression system to clone fibrinogen, fibrinogen variants and subunits thereof, and to the use of recombinant fibrinogen, fibrinogen variants and subunits thereof as tools of research or in medicine, e.g., in diagnostic assays or as therapeutics to treat certain indications. The inventive yeast system produces at least 10 times more recombinant protein than has been possible with other expression systems. Also, the inventive yeast system is easy to adapt to produce large quantities of recombinant protein. Moreover, with the inventive yeast system, recombinant fibrinogen is the principal secretion protein and, thus, is easily purified from the culture medium.</p> |
