发明名称 |
ACETIC ANHYDRIDE ACTIVATION FOR C-TERMINAL PROTEIN SEQUENCING |
摘要 |
A method is provided for C-terminal sequencing of a protein or peptide. An important feature of the method is the formation of an oxazolone moiety at the C-terminus of a protein or peptide by treatment with acetic anhydride under basic conditions followed by conversion of the oxazolone to a thiohydantoin moiety by treatment with thiocyanate under acidic conditions. Yields of thiohydantoin are further enhanced by delivering thiocyanate as the conjugate acid of a sterically hindered alkylammonium cation.
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申请公布号 |
WO9607916(A1) |
申请公布日期 |
1996.03.14 |
申请号 |
WO1995US10975 |
申请日期 |
1995.08.29 |
申请人 |
THE PERKIN-ELMER CORPORATION |
发明人 |
BOYD, VICTORIA, L.;BOZZINI, MERILISA;LOUDON, G., MARC |
分类号 |
C07K1/107;C07K1/12;G01N33/68;(IPC1-7):G01N33/68 |
主分类号 |
C07K1/107 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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