| 摘要 |
<p>The present invention is directed to an improved process for preparing macrocyclic chelating agents and for conjugating the macrocyclic chelating agents to biological molecules. In general, a process for preparing a chelate conjugate is disclosed which comprises purifying a polyaza chelating agent by chromatography on a silica gel column which has been acid washed prior to purification of the chelating agent, functionalizing the chelating agent with thiophosgene to form an activated isothiocyanate polyaza chelating agent which is then conjugated to a biological molecule to form a chelating agent conjugate, followed by contacting the chelating agent conjugate with a metal ion to form a chelate conjugate.</p> |
