Cephalosporin C acylase

摘要

A cephalosporin C acylase with the following characteristics: (a) has ability to catalyze the enzymatic conversion of cephalosporin C, glutaryl 7-ACA, adipyl 7-ACA, succinyl 7-ACA, N-acetylcephalosporin C, N-benzoylcephalosporin C and cephalothin into 7-amino cephalosporanic acid, (b) is composed of alpha -subunit [Molecular weight : 26,000 dalton (SDS-PAGE)] and beta -subunit [Molecular weight : 58,000 dalton (SDS-PAGE)], (c) has N-terminal amino acid sequence of the alpha -subunit : Thr-Met-Ala-Ala-Asn-Thr-Asp-Arg-Ala-Val-Leu-Gln-Ala-Ala-Leu-Pro-Pro-Leu- DNA encoding for the cephalosporin C acylase, an expression vector containing said DNA, and a microorganism transformed with said expression vector are also provided. A process for producing the cephalosporin C acylase, which comprises culturing the transformant in an aqueous nutrient medium, is described.