| 摘要 |
PURPOSE: To unnecessitate complicated fractional molecule operation by mixing a high density lipoprotein(HDL) and dimethyl-β-cyclodextrin in a water liquid to generate turbidity and measuring the turbidity of the mixed liquid. CONSTITUTION: When a sample containing an HDL and dimethyl-β-cyclodextrin (DM-β-CyD) are mixed in a water liquid under a proper condition, the DM-β-CyD reacts with the HDL specifically, generating turbidity. The turbidity of the mixed liquid shows a good relationship to the HDL cholesterol density, so that the HDL cholesterol density can be determined by measuring the turbidity of mixed liquid. First, a DM-β-CyD is dissolved in a proper buffer solution to prepare a reagent. Next, a sample itself, or a sample diluted by a water or physiological saline water as necessary, is treated by the reagent at 10 to 50 deg.C for 1 to 30 minutes. Then, the turvidity of the mixed liquid is measured at the absorbance of 300 to 500nm so as to obtain the HDL cholesterol value based on the resulted value.
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