LOW-PRESSURE CHROMATOGRAPHY COLUMN WITH AUTOMATICALLY MOVABLE LID

摘要

The invention relates to a low-pressure chromatography column 1 having a diameter of more than 30 cm, essentially consisting of a vertical cylinder 13 having a base plate 12, a lower sintered disk 3 which is arranged above the base plate 12, an upper sintered disk 16 which is fastened to the underside of a lid designed as a mobile plunger 4, wherein the low-pressure chromatography column 1 has controllable elements 5 for raising or lowering the plunger 4, which are connected to the plunger 4, wherein the controllable elements 5 are equipped with sensors 6 for determining the vertical position and horizontal placement of the plunger 4, and wherein the sensors 6 are electrically connected to a control unit 10 for the controllable elements 6. With the aid of an optical observation device (endoscope), the plunger/gel material can be adjusted exactly over the entire positioning range of the plunger. The advantages which can be obtained using the column are manifested, on the one hand, by simple and rapid production of the packing and, on the other hand, it has surprisingly been found that purer products could be obtained with such packing than hitherto. The low-pressure chromatography column according to the invention is particularly suitable for purifying insulin and precursors thereof.