发明名称 |
Polypeptide from Humicola insolens possessing protein disulfide isomerase activity gene encoding the same |
摘要 |
A highly thermostable polypeptide possessing protein disulfide isomerase (PDI) activity, a gene coding for the polypeptide and a process for producing the polypeptide are provided. The polypeptide possessing PDI activity is characterized by A) having a capability of catalyzing a disulfide exchange in proteins, B) recognizing mainly ribonuclease A as a substrate, C) having a suitable active temperature of 20 DEG to 70 DEG C., D) being stable at a pH value of 6 to 9, and E) having a molecular weight of about 60,000 to 62,000. Since it has a higher thermostability and exhibits a stable activity in a wider dithiothreitol concentration range as compared with the conventional PDI, it is possible to provide a novel enzyme active protein which can be advantageously used for a refolding reaction of certain proteins. Further, a process which enables the polypeptide possessing PDI activity to be efficiently produced using Humicola insolens or a transformant transformed with an expression vector containing the above-described gene is also provided.
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申请公布号 |
US5496719(A) |
申请公布日期 |
1996.03.05 |
申请号 |
US19930068395 |
申请日期 |
1993.05.27 |
申请人 |
KABUSHIKI KAISHA TOYOTA CHUO KENKYUSHO |
发明人 |
YAMADA, YUKIO;ASAMI, OSAMU;SUGIYAMA, HIDEHIKO;IDEKOBA, CHIE;HOSHINO, FUMIHIKO;HIRAI, MASANA;KAJINO, TSUTOMU;IMAEDA, TAKAO;SARAI, KIYOKO |
分类号 |
C12N9/90;(IPC1-7):C12N9/90;C12N9/00;A61K38/00;C07K1/00 |
主分类号 |
C12N9/90 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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