| 摘要 |
PURPOSE: To obtain the subject new compound as a specific derivative from Cypridinacea luciferin, capable of making itself luminous as a substrate for saccharide hydrolase such asα-D-galactosidase, thus useful for determination, immunoassay, etc., of saccharide hydrolase. CONSTITUTION: This new Cypridinacea luciferin derivative is expressed by formula I(R<1> and R<2> are each H, a 1-20C alkyl, 6-20C aryl or 7-19C arylalkyl; R<3> is a 1-5C alkyl or alkoxy; (n) is an integer of 0-5), which can make itself luminous as a substrate for saccharide hydrolase such asα-D-galactosidase, thus is useful for saccharide hydrolase deteronination, enzyme immunoassay by luminescent technique. The new compound is obtained by reaction between an imidazopyrazine derivative of formula II and a saccharide derivative of formula III (X is a halogen; R<4> is a 1-7C acyl) in the presence of silver trifluoromethanesulfonate and disodium phosphate to form a new intermediate of formula IV, which is then subjected to solvolysis in the presence of an alkali.
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