发明名称 |
Assay method for hemicellulases using a colored substrate |
摘要 |
The present invention provides an assay method for hemicellulases comprising a) directly dyeing, using a reactive dye, an insoluble natural product, or a modified form of a natural fibre material; and b) adding the enzyme to the dyed product produced in step a) and, after a specific incubation period, separating the liquid component from the insoluble dyed product, e.g. by a simple filtration, and determining the amount of dyestuff liberated in the the separated solution by spectrophotometric means. A combined mixing and dispensing device for use in the method of the present invention is also provided.
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申请公布号 |
US5496707(A) |
申请公布日期 |
1996.03.05 |
申请号 |
US19940221649 |
申请日期 |
1994.04.05 |
申请人 |
CIBA-GEIGY CORPORATION;GENENCOR INTERNATIONAL, INC. |
发明人 |
FREIERMUTH, BEAT;WERTHEMANN, DIETER;GAERTNER, ALFRED;FISK, SPENCER |
分类号 |
C12Q1/34;(IPC1-7):C12Q1/34;C07H1/00 |
主分类号 |
C12Q1/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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