发明名称 |
MATRIX WITH CELLS ADHERENTLY BOUNDED TO IT, AND THE PROCESS FOR THE PRODUCTION OF VIRUSES/VIRUS ANTIGENES |
摘要 |
<p>A matrix or substrate bears adherently bound human or animal cells infected with a virus. It has been shown that surface dependent cells useful for virus multiplication remain adherently bound to a matrix even when infected with a virus, produce virus antigens over a relatively long period of time and release them in the culture medium. In order to produce early Summer meningo-encephalitis (FSME) virus antigens by cultivating the FSME virus in cell cultures, a surface dependent permanent cell line, preferably the vero-cell line ATTC CCL 81, is inoculated with the FSME virus and the cells are bound to substrates and kept in a non lytic serum free system in conditions that ensure cellular growth, so that antigens are produced. The antigen-containing medium is then separated from the substrate bound cells and processed in a known manner by concentration, inactivation and purification until a galenically acceptable composition is obtained.</p> |
申请公布号 |
HRP921354(A2) |
申请公布日期 |
1996.02.29 |
申请号 |
HR1992P001354 |
申请日期 |
1992.11.25 |
申请人 |
IMMUNO AKTIENGESELLSCHAFT |
发明人 |
MUNDT, WOLFGANG;BARRETT, NOEL;DORNER, FRIEDRICH;EIBL, JOHANN |
分类号 |
A61K39/00;A61K35/76;A61K39/12;C12N5/00;C12N5/06;C12N5/10;C12N7/00;C12P21/00;C12R1/91;(IPC1-7):C12N5/10 |
主分类号 |
A61K39/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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