| 摘要 |
An analytical process for purifying phthalyl amidase from Xanthobacter agilis is presented. The process comprises the sequential steps of obtaining a cell free extract, anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, hydroxylapatite chromatography, and a second anion exchange chromatography. The resulting amidase is about 95 % purity as determined by SDS electrophoresis. A preparative process is also presented which comprises the sequential steps of anion exchange chromatography of the cell free extract followed by hydroxylapatite chromatography. |
