发明名称 |
Ligation wity hammerhead ribozymes |
摘要 |
The claimed invention is drawn to a method for ligation of an enzymatic nucleic acid molecule having a hammerhead motif to a separate nucleic acid molecule. The method includes use of ligation enhancing conditions in which ligation is enhanced at least two fold over that observed in a medium containing only Mg2+ as a divalent cation at 20 DEG C. |
申请公布号 |
AU2957095(A) |
申请公布日期 |
1996.02.16 |
申请号 |
AU19950029570 |
申请日期 |
1995.06.29 |
申请人 |
RIBOZYME PHARMACEUTICALS, INC. |
发明人 |
DAVID M. LONG JR.;OLKE C UHLENBECK;KLEMENS J HERTEL |
分类号 |
C12N15/113 |
主分类号 |
C12N15/113 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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