| 摘要 |
The invention relates to a process for releasing intracellular heterolog proteins in the Saccharomyces Cerevisiae yeast based on the osmotic sensitivity which confers the mutation slt2 at the temperature of 37 DEG C. Said osmotic sensitivity may be used for releasing the intracellular content either by change of temperature of the culture from 24 DEG to 37 DEG C, or by transfer of grown cells in an osmotically stabilized medium at said second temperature to a non-stabilized medium. In both cases, the cells undergo a lysis and release their intracellular content and, after centrifugation to eliminate the cellular rests, a preparation of proteins is obtained which is appropriate to initiate a process of purification of the protein of interest. This process results in a cost reduction since the use of equipment is avoided and the preparation of proteins which is obtained contains less impurities than that which is obtained through other processes. |
