PCR primers for detection of legionella species and methods for controlling visual intensity in hybridization assays

摘要

PCT No. PCT/US91/09688 Sec. 371 Date May 27, 1993 Sec. 102(e) Date May 27, 1993 PCT Filed Dec. 19, 1991 PCT Pub. No. WO92/11273 PCT Pub. Date Jul. 9, 1992.This invention provides for superior nucleic acid primers for amplification of select target regions of the genome of the genus Legionella. The invention facilitates detection of pathogenic and nonpathogenic forms of this genus. The invention further provides for processes for using the primers in template dependent nucleic acid polymerase extension reactions to amplify select target regions. Kits for the use of these primers are also provided. This invention further provides for methods of controlling the intensity of visual signal for detection of duplex formation in nucleic acid hybridization assays under high stringent conditions. This method involves the blending of different capture probes onto a solid support.