发明名称 |
Uses of green-fluorescent protein |
摘要 |
This invention provides a cell comprising a DNA molecule having a regulatory element from a gene, other than a gene encoding a green-fluorescent protein operatively linked to a DNA sequence encoding the green-fluorescent protein. This invention also provides a method for selecting cells expressing a protein of interest which comprises: a. introducing into the cells a DNAI molecule having DNA sequence encoding the protein of interest and DNAII molecule having DNA sequence encoding a green-fluorescent protein; b. culturing the introduced cells in conditions permitting expression of the green-fluorescent protein and the protein of interest; and c. selecting the cultured cells which express green-fluorescent protein, thereby selecting cells expressing the protein of interest. Finally, this invention provides various uses of a green-fluorescent protein.
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申请公布号 |
US5491084(A) |
申请公布日期 |
1996.02.13 |
申请号 |
US19930119678 |
申请日期 |
1993.09.10 |
申请人 |
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK;WOODS HOLE OCEANOGRAPHIC INSTITUTION |
发明人 |
CHALFIE, MARTIN;PRASHER, DOUGLAS |
分类号 |
C07K14/435;C12N15/65;C12Q1/68;(IPC1-7):C12N9/02;C12N5/00;C12P21/06;C07H19/00 |
主分类号 |
C07K14/435 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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