发明名称 |
PROCESS FOR PREPARING VIRUSES AND VIRAL VECTORS |
摘要 |
The invention concerns a process for preparing viruses and viral vectors by the transfection of mammal cells with complexes of viral DNA and a polycation, which is optionally conjugated with a ligand for the target cell, in the presence of an endosomolytic agent. The process affords particular advantages for the production of recombinant retroviruses, adenoviruses and adenoassociated viruses in the cell, permitting high virus titres in packing cell lines and in primary cells. The area of application is above all gene therapy.
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申请公布号 |
CA2194465(A1) |
申请公布日期 |
1996.02.08 |
申请号 |
CA19952194465 |
申请日期 |
1995.07.13 |
申请人 |
BOEHRINGER INGELHEIM INTERNATIONAL GMBH |
发明人 |
VON RUEDEN, THOMAS;COTTEN, MATTHEW;WAGNER, ERNST;ZATLOUKAL, KURT;LEHRMANN, HEIKE;PANZENBOECK, BIRGIT |
分类号 |
C12N15/09;A61K47/48;C07K14/015;C07K14/075;C12N7/00;C12N9/50;C12N15/86;(IPC1-7):C12N15/86;A61K48/00;A61K31/70 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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