ANALOG OF HAEMOPHILUS HIN47 WITH REDUCED PROTEASE ACTIVITY

摘要

The invention concerns isolated and purified analogs of Haemophilus influenza Hin47 protein with decreased protease activity (of less than 10 % of that of the natural protein) but preferably retaining substantially the same immunogenic properties as natural Hin47. Preferred analogs have mutations at Ser197, His91 and/or Asp121 positions and are possibly used as chimeric proteins with other immunogenic molecules. Also disclosed are nucleic acid encoding said analogs, recombinant plasmids and transformed host cells containing said modified genes, immunogenic compositions containing Hin47 analogs or their nucleic acid and their use for prophylactic, vaccine or diagnostic purposes.