| 摘要 |
A nucleotide sequence amplification method wherein a polynucleotide containing the sequence is contacted with oligonucleotide primers, nucleoside triphosphates and/or deoxynucleoside triphosphates containing the various purine and pyrimidine bases needed to synthesise the amplification product, and an enzyme system having DNA polymerase and/or RNA polymerase and/or reverse transcriptase activity, under conditions enabling the sequence of interest to be amplified in a per se known manner. One of the nucleoside triphosphates and/or deoxynucleoside triphosphates is present either in a modified form or in both a modified form and an unmodified form, and, in said modified form, the purine or pyrimidine base is substituted by at least one methyl, hydroxymethyl or acetyl grouping.
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