| 摘要 |
<p>PURPOSE:To obtain compound useful as a hapten for preparing an antibody in measuring a vitamin D derivative. CONSTITUTION:A compound of formula I (R1 and R2 are each H or CO (CH2)nCO2H; (n) is 1-5 with the proviso that R1 and R2 are not H at the same time). The compound is obtained by protecting hydroxyl groups except the 25-position of a compound of formula II, reacting the resultant substance with a compound of the formula R3 (CH2)nCOOH (R3 is carboxyl, etc.; (n) is 1-5) or its reactive derivative to give a compound of formula III (A1, A2 and A3 are each a protecting group) and deprotecting the resulting substance. The compound of formula I is used as a hapten to give an antibody. A very small amount of vitamin D can be measured by a method such as enzyme immunoassay or radioimmunoassay using the antibody.</p> |
