发明名称 |
METHOD FOR PRODUCING HIRUDIN |
摘要 |
The hirudin expression vector pYEGa-HIR5 (KCTC 8518P) containing hirudin gene (I) consists of linked promoter GAL1-GAL10, alpha factor of yeasts (a on plasmid means alpha), peptide sequence for leu-asp-lys-arg which is hydrolyzed by KEX2 as operator endopeptidase and hirudin gene. The other plasmid pYEGa-HIR525 (KCTC 8519BP) (II) consists of GAL10 as promoter rather than GAL1-GAL10 of (I). (I) and (II) are useful for yeast expression to solve the problems which in E. coli the expressed hirudin is hydrolysed and the N-terminal methionine modified hirudin does not have the activity.
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申请公布号 |
KR960001818(B1) |
申请公布日期 |
1996.02.05 |
申请号 |
KR19920011007 |
申请日期 |
1992.06.24 |
申请人 |
KIST |
发明人 |
LEE, SANG - KI;CHOE, EUI - SUNG;SONN, JUNG - HOON;LEE, SANG - KWON |
分类号 |
C12N15/15;(IPC1-7):C12N15/15 |
主分类号 |
C12N15/15 |
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地址 |
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