MICRO-ORGANISM FOR PRODUCING L-LYSINE

摘要

The new KFCC 10679 having L-proline derivative resistance for producing L-lysine consists of (1) treating Corynebacterium glutamicum KFCC 10672 with NTG and selecting the mutants with the medium containing 1.5% L-azetidine-2-carboxylate as proline derivatives at 30-32 deg. C for 4-5 days to isolate the mutant(I), and (2) culturing (I) in the medium containing sucrose 10%, ammonium sulfate 2.7%, corn steep liquor 10%, biotin, thiamin, pantothenate as vitamins and lucien, threonine, methionine as amino acid at pH 7.0-7.2 for 84 hours. The final concentration of L-lysine is 45.1 gram/liter. The proline derivative is osmoprotecting agent for the obtained mutant.