| 摘要 |
<p>PURPOSE:To detect and quantitatively determine non-A, non-B hepatitis virus CORE structural protein in a specimen by producing a monoclonal antibody, which performs specific bonding to an antigen determination region, from a hybridoma cell line. CONSTITUTION:A specimen (for example, blood serum) together with polyethylene glycol is subjected to centrifuging and precipitate thereof is alkaline- processed to concentrate and decompose a virus particle. A mouse is immunized by non-A, non-B hepatitis virus CORE structural protein of this virus particle, and a lymphatic corpuscle derived from this and a myeloma cell are subjected to blending so as to obtain a hybridoma cell line. A monoclonal antibody produced by culturing this cell line is specifically bonded to an antigen determinination region on the non-A, non-B hepatitis virus CORE structural protein. Accordingly, by using this monoclonal antibody, the non-A, non-B hepatitis virus CORE structural protein can be detected and quantitatively determined.</p> |
