A method of detecting tropical fever, tertian malaria, quartan malaria and Plasmodium ovale by using primers represented by formulae (1), (3) through (10). This method allows all of the plasmodia that infect the human being to be detected readily and rapidly at a high sensitivity and differentiated accurately, thus enabling malaria treatment or large-scale mass examination in the area where malaria is prevalent. 5'AAGTCATCTTTCGAGGTGAC3' (4> 5'GAATTTTCTCTTCGGAGTTTA3' (5> 5'GAGACATTCTTATATATATG3' (3> 5'GAAAATTCCTTTCGGGGA3' (1> 5'CGACTAGGTGTTGGATGA3' (6> 5'GAACGAAAGTTAAGGGAGT3' (7> 5'ACTGAAGGAAGCAATCTAA3' (8> 5'TCAGATACCGTCGTAATCTT3' (9> 5'CCAAAGACTTTGATTTCTCAT3' (10)
