| 摘要 |
PURPOSE:To obtain a material composed of a substrate and a polymer compound supported on the substrate and having a carbodiimide group, capable of easily immobilizing a biologically active substance and useful e.g. as immobilized enzyme, antibody or antigen or a diagnostic having immobilized nucleic acid. CONSTITUTION:This material for immobilizing a biologically active substance is produced by reacting a substrate consisting of a 96-hole microplate made of polystyrene with a diisocyanate compound such as 4,4'-dicyclohexylmethane diisocyanate and a diamine such as 1,4-diaminobutane, adding a solution of a polymer compound having a carbodiimide group and prepared by treating with a carbodiimidation catalyst such as 3-methyl-1-phenylphospholene-1-oxide and incubating the mixture at 60 deg.C for 1hr. An aqueous solution of a biologically active substance such as enzyme, hormone, antigen, antibody, hapten, peptide or DNA is poured into the well and treated at 37 deg.C for 2hr to immobilize the active substance to the material. |
