| 摘要 |
PURPOSE:To obtain a new enzyme gene coding a nitrate reducing enzyme of a plant of the genus Populus having a specific amino acid sequence and useful for the creation of plant, especially tree having strengthened absorbing and cleaning power of air-polluting nitrogen oxides. CONSTITUTION:This new nitrate-reducing enzyme gene of a plant of the genus Populus codes a nitrate-reducing enzyme of a plant of the genus Populus having an amino acid sequence of the formula and is useful for the creation of plant, especially tree having strengthened absorbing and cleaning power of nitrogen oxides known as air-polluting substances. This nitrate-reducing enzyme gene is produced by continuously irradiating poplar grown to about 20cm on vermiculite with light of 10,000 lux for 2 days, adding 60mM KNO3, extracting mRNA, synthesizing cDNA using the mRNA as a template, preparing a cDNA library by conventional method, screening the library using a probe and recovering the DNA from a positive clone. |
