发明名称 |
PROCESSES FOR THE PURIFICATION OF HUMAN RECOMBINANT DECORIN AND THE DETECTION OF GUANIDINIUM IONS |
摘要 |
The present invention is directed to a process for the production of substantially pure human recombinant decorin which involves the combination of three separate stages characterized by contacting decorin-containing cell culture medium with (1) a first strong anionic exchange resin; then with (2) a hydrophobic interactive chromatographic resin; and finally with (3) a second strong anionic exchange resin. By using a combination of steps, and certain reagents, in particular a 2.4 to 3 molar GuHCl solution to elute decorin from a hydrophobic interactive column, the process of this invention provides a more convenient and reproducible process for purifying human recombinant decorin. The invention also provides a process for detecting the presence of guanidinium ions in a sample solution. |
申请公布号 |
CA2194566(A1) |
申请公布日期 |
1996.01.25 |
申请号 |
CA19952194566 |
申请日期 |
1995.07.07 |
申请人 |
LA JOLLA CANCER RESEARCH FOUNDATION |
发明人 |
CRAIG, WILLIAM S.;HARPER, JOHN R.;HERNANDEZ, SAM D.;KOSTEL, PAUL J.;PARKER, JONATHAN R.;VEDVICK, THOMAS S. |
分类号 |
G01N30/02;A61K38/00;A61P35/00;A61P43/00;B01D41/04;C07K14/00;C07K14/47;C07K14/78;C12N15/09;C12Q1/00;G01N30/06;G01N30/26;G01N30/46;G01N30/88;G01N30/96;G01N33/53;G01N33/68;(IPC1-7):C07K14/47;C07K1/18;G01N27/04;G01N27/06;C07K1/20 |
主分类号 |
G01N30/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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