DRY-TYPED MULTILAYER ANALYSIS ELEMENT TO ASSAY TRANSAMINASE

摘要

A dry multilayer analytical element for assaying transaminases having, in the following order, a spreading layer, a reagent layer and a support and the element comprises: a) a transaminase substrate; b) reagents that generate pyruvate in the presence of a transaminase and the transaminase substrate; c) pyruvate oxidase and cofactors for pyruvate oxidase; d) reagents that generate a color in proportion to a concentration of hydrogen peroxide; characterized in that: e) the spreading layer contains the transaminase substrate and a material selected from the group consisting of polymer beads, barium sulfate, titanium dioxide and mixtures thereof; f) the reagent layer contains the pyruvate oxidase; and g) at least one layer separate the spreading layer from the reagent layer.