发明名称 |
Method of carrying out an immuno-assay in a multi-phase system |
摘要 |
The present invention relates to a method of carrying out an immunoassay in a multiphase system. A sample containing an analyte is brought into contact with a receptor A and a tracer. The analyte can either form a complex with the tracer, or counteract the formation of a complex of receptor A and tracer by competing with the tracer for binding to receptor A, or counteract the formation of a complex of receptor A and tracer by competing with receptor A. Receptor B is added and the signal is determined. In this method, receptor A and receptor B are suitably immobilized, ensuring that the tracer either cannot enter into any binding involving the simultaneous participation of receptors A and B or can enter into such a binding to only such a slight extent that it is nevertheless possible to detect and differentiate differing analyte concentrations. |
申请公布号 |
AU2924395(A) |
申请公布日期 |
1996.01.19 |
申请号 |
AU19950029243 |
申请日期 |
1995.06.23 |
申请人 |
BEHRINGWERKE AKTIENGESELLSCHAFT |
发明人 |
STEPHAN NEUENHOFER;REINHARD KASMARKER |
分类号 |
G01N33/542;C12N9/96;C12Q1/68;G01N33/53;G01N33/532;G01N33/543;G01N33/566;G01N33/574;G01N33/577;G01N33/58 |
主分类号 |
G01N33/542 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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