O-GLYCOSYLATED AUTHENTIC IGF-I AND TRUNCATED VARIANTS THEREOF, A METHOD OF PREPARATION THEREOF AND PHARMACEUTICAL COMPOSITIONS
摘要
The invention relates to O-glycosylated authentic IGF-I, having three or more mannose residues to the Ser69 amino acid of the IGF-polypeptide chain or being truncated and having two mannose residues to the Thr29 amino acid of the truncated IGF-polypeptide chain. The O-glycosylated IGF-I according to the invention have substantially no insulin receptor affinity, which makes them valuable for use in therapeutic treatment. The invention also relates to a method of obtaining IGF-I as defined above by expressing IGF-I in yeast cells and isolating the claimed O-glycosylated IGF-I from the medium and to pharmaceutical composition containing these IGF-I together with a pharmaceutically acceptable carrier, diluent or excipient. It also relates to the use of the O-glycosylated IGF-I in the preparation of a medicament for the treatment of e.g. growth deficiency and insulin resistant states.
申请公布号
WO9601275(A1)
申请公布日期
1996.01.18
申请号
WO1995SE00774
申请日期
1995.06.22
申请人
PHARMACIA AB;ERIKSSON, ROLF;JOHANSSON, STIG;ROENNHOLM, HARRIET;SKOTTNER, ANNA;SONESSON, KARIN
发明人
ERIKSSON, ROLF;JOHANSSON, STIG;ROENNHOLM, HARRIET;SKOTTNER, ANNA;SONESSON, KARIN