METHOD AND SYSTEM FOR CULTIVATING CELLS PARTICULARLY MACROPHAGES

摘要

A method of cultivating cells present in suspension by placing the cells, one or more reagents, and a culture medium in separate sterile containers connected together and hermetically sealed by sterile tubings having fluid flow control devices which may be manually opened and closed, and opening and closing the fluid flow control devices as required in order to transfer the contents of one container to another, while isolating the container contents from the external environment. The method is particularly useful for cultivating macrophages in a completely controlled atmosphere hermetically sealed from the outside atmosphere.