发明名称 Treatment of paraffin embedded tissue for gene analysis
摘要 Rapid and easy preparation of a sample for a gene analysis or high-purity nucleic acid suitable for gene amplification especially, for example, by the PCR method is made possible by a process for treating a paraffin-embedded tissue sample to be used for a gene analysis, which comprises heating an aqueous suspension containing a surfactant having a protein-denaturational action and a deparaffinized tissue sample obtained from a paraffin-embedded tissue sample at 60 DEG C or higher, or the above-mentioned process which further comprises reacting the heat-treated aqueous solution with a protease, or the above-mentioned process which further comprises reacting the heat-treated aqueous solution with a protease, mixing the resulting reaction solution with a solution containing an organic compound having a protein-denaturational action other than the aforesaid surfactant, and precipitating nucleic acid from the resulting reaction solution by addition of an alcohol.
申请公布号 EP0692533(A1) 申请公布日期 1996.01.17
申请号 EP19950304717 申请日期 1995.07.05
申请人 WAKO PURE CHEMICAL INDUSTRIES LTD 发明人 WANG, LU;HIRAYASU, KAZUNARI, C/O OSAKA KENKYUSHO
分类号 C12Q1/68 主分类号 C12Q1/68
代理机构 代理人
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